A simple, rapid, precise, stable and accurate liquid chromatographic method (HPLC) was developed for the simultaneous estimation of Clindamycin phosphate (CLI), Clotrimazole (CLO) and Tinidazole (TIN) in pharmaceutical dosage form. A Kromasil C18 column (150 MM ×4.6 MM, 5 µm) in isocratic mode using ph 2.5 ±0.01 phosphate buffer and methanol in the ratio of 40:60 as a mobile phase was used. The flow rate was maintained at 1 mL/min. The detection was carried out at 210 nm. The column temperature was maintained at 40 oC. The retention time was found to be 2.7 min, 3.9 min and 8.5 min TIN, CLI and CLO respectively. The method was validated for linearity, accuracy, precision and robustness. The assay was linear over the range of 75-350 µg/ml. The average recovery of TIN, CLI and CLO was found to be 99.87±0.64 %, 100.61±1.02 % and 100.03±0.84 % respectively. The percentage relative standard deviation (%RSD) was found to be less than 2 % in precision study for each drug. The proposed method was successfully applied for the quantitative determination of CLI, CLO and TIN in pharmaceutical dosage form.
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